Issue 78, 2015
From the journal:

Chemical Communications

A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue†

Zhiqing Yang,‡a   Wenjing Jiang,‡a   Fei Liu,b   Yunlei Zhou,a   Huanshun Yin*a  and  Shiyun Ai*a  

* Corresponding authors

a College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, P. R. China
E-mail: yinhs@sdau.edu.cn, ashy@sdau.edu.cn
Fax: +86-538-8242251

b The Tumor Center of Taian City Central Hospital, Taian, Shandong, P. R. China

Abstract

A novel electrochemical immunosensor was fabricated for the quantitative detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA based on anti-5-hmC antibody, biotin functionalized phos-tag and avidin functionalized alkaline phosphatase. It is demonstrated that the levels of 5-hmC are dramatically reduced in human breast cancer tissue compared with those in normal tissue.

Graphical abstract: A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue

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Article information

DOI
https://doi.org/10.1039/C5CC05921F
Article type
Communication
Submitted
16 Jul 2015
Accepted
12 Aug 2015
First published
12 Aug 2015

Chem. Commun., 2015,51, 14671-14673

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A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue

Z. Yang, W. Jiang, F. Liu, Y. Zhou, H. Yin and S. Ai, Chem. Commun., 2015, 51, 14671 DOI: 10.1039/C5CC05921F

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