Issue 13, 2015

A practical kit for micro-scale application of the ceiling crystallisation method

Abstract

We designed a kit to facilitate the optimisation of protein crystal growth by means of the ceiling crystallisation method. This ceiling kit allows for diffusion-limited crystal growth with a total volume down to 10 μl per trial and air-tight sealing for any type of protein sample. The ceiling crystals grow to sufficient size for diffraction experiments. In this highlight, we fully describe the features of this kit and how easily it can be used to yield protein crystals.

Graphical abstract: A practical kit for micro-scale application of the ceiling crystallisation method

Article information

Article type
Highlight
Submitted
03 Sep 2014
Accepted
05 Jan 2015
First published
05 Jan 2015

CrystEngComm, 2015,17, 2602-2605

Author version available

A practical kit for micro-scale application of the ceiling crystallisation method

A. Adawy, W. Corbeek, E. de Ronde, W. J. P. van Enckevort, W. J. de Grip and E. Vlieg, CrystEngComm, 2015, 17, 2602 DOI: 10.1039/C4CE01814A

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