Issue 10, 2015

Suppressive effects of saponin-enriched extracts from quinoa on 3T3-L1 adipocyte differentiation

Abstract

This study was performed to investigate the effect of quinoa saponins (QS) on the differentiation of 3T3-L1 preadipocytes. QS inhibited triglyceride (TG) accumulation in the mature adipocytes, evidenced by oil-red O staining and intracellular quantification. Real time-PCR analysis and western blot analysis showed that QS significantly down-regulated the mRNA and protein expression of key adipogenic transcription factors, peroxisome proliferator-activated receptor γ (PPARγ), and CCAAT/enhancer-binding protein alpha (C/EBPα), however, they had no significant effect on CCAAT/enhancer-binding protein beta (C/EBPβ) and CCAAT/enhancer-binding protein delta (C/EBPδ) which are the upstream regulators for adipogenesis compared with mature adipocytes. QS also reduced mRNA and protein expression of sterol regulatory element-binding protein-1c (SREBP-1c) related to the late stage of adipogenesis. Furthermore, lipoprotein lipase (LPL), adipocyte protein 2 (aP2) and glucose transporter 4 (Glut4), as adipocyte specific genes, were decreased in mature adipocytes by QS treatment. These findings indicate that QS are capable of suppressing adipogenesis and therefore they seem to be natural bioactive factors effective in adipose tissue mass modulation.

Graphical abstract: Suppressive effects of saponin-enriched extracts from quinoa on 3T3-L1 adipocyte differentiation

Article information

Article type
Paper
Submitted
15 Jun 2015
Accepted
18 Jul 2015
First published
21 Jul 2015

Food Funct., 2015,6, 3282-3290

Suppressive effects of saponin-enriched extracts from quinoa on 3T3-L1 adipocyte differentiation

Y. Yao, Y. Zhu, Y. Gao, Z. Shi, Y. Hu and G. Ren, Food Funct., 2015, 6, 3282 DOI: 10.1039/C5FO00716J

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