Horseradish peroxidase-immobilized magnetic mesoporous silica nanoparticles as a potential candidate to eliminate intracellular reactive oxygen species

Abstract

Horseradish peroxidase-immobilized magnetic mesoporous silica nanoparticles (MMSNs–HRP) have been synthesized by a NHS/EDC coupling between the amino groups of horseradish peroxidase (HRP) and the carboxyl groups on the MMSNs surface. It is found that the immobilized HRP on MMSNs still retain high activity and the MMSNs–HRP can eliminate the reactive oxygen species (ROS) in Chinese hamster ovary (CHO) cells induced by the addition of H₂O₂ aqueous solution. Further, the fluorescent MMSN–HRP–CD nanoparticles have been prepared by attaching biocompatible, fluorescent carbon dots (CDs) to MMSNs–HRP. We have also investigated the effect of an applied magnetic field on cellular uptake of MMSNs–HRP–CDs and found that the internalization of MMSNs–HRP–CDs by CHO cells could be enhanced within 2 hours under the magnetic field. This work provides us with a novel and efficient method to eliminate ROS in living cells by using HRP-immobilized nanoparticles.