Issue 8, 2015
From the journal:

RSC Advances

A highly selective probe for UDP-glucuronosyltransferase 2B7 (UGT2B7) in human microsomes: isoform specificity, kinetic characterization, and applications†

Xiang-Ge Tian,‡a   Chao Wang,‡a   Guang-Bo Ge,‡b   Jing Ning,ab   Chun-Zhi Ai,b   James Y. Hong,c   Yong-Xv Cai,a   Xiao-Kui Huo,a   Jie Hou,a   Ke-Xin Liu,a   Hong-Zhi Sund  and  Xiao-Chi Ma*ad  

* Corresponding authors

a College of Pharmacy, Research Institute of Integrated Traditional and Western Medicine, Pharmacokinetic and Drug Transport Key Laboratory, Dalian Medical University, Dalian, China
E-mail: maxc1978@163.com

b Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

c Department of Biopharmaceutical Sciences, University of Illinois, Chicago, USA

d Joint Center for Translational Medicine, First Affiliated Hospital of Liaoning Medical University, Jinzhou, China

Abstract

3-Epideacetycinobufagin (EDCB) was found to be a highly isoform-specific probe substrate for 3-glucuronidation mediated by UDP-glucuronosyltransferase 2B7 (UGT2B7). The probe reaction was well-characterized, and our results strongly suggest that EDCB can be used specially to measure the catalytic activity of UGT2B7 in biological samples.

Graphical abstract: A highly selective probe for UDP-glucuronosyltransferase 2B7 (UGT2B7) in human microsomes: isoform specificity, kinetic characterization, and applications

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Article information

DOI
https://doi.org/10.1039/C4RA09819F
Article type
Communication
Submitted
05 Sep 2014
Accepted
26 Nov 2014
First published
27 Nov 2014

RSC Adv., 2015,5, 5924-5927

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A highly selective probe for UDP-glucuronosyltransferase 2B7 (UGT2B7) in human microsomes: isoform specificity, kinetic characterization, and applications

X. Tian, C. Wang, G. Ge, J. Ning, C. Ai, J. Y. Hong, Y. Cai, X. Huo, J. Hou, K. Liu, H. Sun and X. Ma, RSC Adv., 2015, 5, 5924 DOI: 10.1039/C4RA09819F

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