On-line microextraction of moxifloxacin using Fe3O4 nanoparticle-packed in-tube SPME

Abstract

In the current study, an in-tube solid-phase microextraction (SPME) approach based on a Fe₃O₄ nanoparticle-packed tube for microextraction and detection of moxifloxacin has been reported. In this regard, the Fe₃O₄ nanoparticles (NPs) were prepared by a batch synthesis and were introduced into a stainless steel tube by a syringe. Afterward, a strong magnet was placed around the tube, so that the Fe₃O₄ NPs were retained in the tube and the tube was used in the in-tube SPME-HPLC for the analysis of moxifloxacin in urine samples. Sodium dodecyl sulfate (SDS) was used as bridging agent between the Fe₃O₄ NPs and moxifloxacin. The operational conditions such as the uptake and release times, flow rate and pH of the solution were investigated and optimized. According to our findings, the linear range and the detection limit (S/N = 3) were 0.1–5000 and 0.03 μg L⁻¹, respectively. Simplicity and facile automation, rapid analysis, high sensitivity, wide linear range, and fully sorbent collection after analysis are the main advantages of the method.