Issue 106, 2015
From the journal:

RSC Advances

Genetically encoded fluorescence screening probe for MgrA, a global regulator in Staphylococcus aureus†

Yujie Wang,a   Hong Zhang,b   Qingzhou Zhang,a   Yujie Liang,a   Lin Ma,a   Hongsheng Tan,b   Yuanzhi Lao,*b   Hongxi Xu*b  and  Zigang Li*a  

* Corresponding authors

a School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, Guangdong, P. R. China
E-mail: lizg@pkusz.edu.cn

b School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China
E-mail: xuhongxi88@gmail.com, laurence_ylao@163.com

Abstract

Herein, we construct a novel cell-based fluorescence response screening system based on a single insertion of circularly permuted yellow fluorescent protein (cpYFP) to reflect the protein conformational change caused by MgrA–small molecules interaction. And this screening platform was applied for screening of Chinese herb extracts with two extracts identified from 351.

Graphical abstract: Genetically encoded fluorescence screening probe for MgrA, a global regulator in Staphylococcus aureus

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Article information

DOI
https://doi.org/10.1039/C5RA11455A
Article type
Communication
Submitted
16 Jun 2015
Accepted
07 Oct 2015
First published
08 Oct 2015

RSC Adv., 2015,5, 87216-87220

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Genetically encoded fluorescence screening probe for MgrA, a global regulator in Staphylococcus aureus

Y. Wang, H. Zhang, Q. Zhang, Y. Liang, L. Ma, H. Tan, Y. Lao, H. Xu and Z. Li, RSC Adv., 2015, 5, 87216 DOI: 10.1039/C5RA11455A

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