Kinetic study of DNA hybridization on DNA-modified gold nanoparticles with engineered nano-interfaces

Abstract

Colorimetric DNA sensing systems using DNA-modified gold nanoparticles (GNPs) rely on DNA hybridization events on the GNP. We report a systematic study of DNA hybridization kinetics on GNPs with different surface modifications using probe DNA and different molecular weight PEGs. The DNA hybridization kinetics are extremely sensitive not only to probe DNA density but also to the molecular weight of PEG.