Solution-blown core–shell hydrogel nanofibers for bovine serum albumin affinity adsorption

Abstract

In this work, nylon 6 core–chitosan/poly(vinyl alcohol) (PVA) shell hydrogel nanofibers (NCNFs) were fabricated by coaxial solution blowing. The hydrogel fibers were 80–650 nm in diameter with smooth surfaces. These fibers were distributed randomly and formed three-dimensional mats. Cibacron Blue F3GA (CB) was then immobilized onto the membrane surfaces for subsequent protein affinity adsorption. The amount of PVA in the shell greatly influenced CB content and bovine serum albumin (BSA) adsorption. The highest BSA adsorption capacity achieved by the NCNF membranes with immobilized CB was 379.43 mg g⁻¹. The results showed that NCNFs combine the large capacity of hydrogels and the high flux of nanofibrous mats for affinity adsorption.