A colorimetric and ultrasensitive immunosensor for one-step pathogen detection via the combination of nanoparticle-triggered signal amplification and magnetic separation

Abstract

We develop a visual immunosensor for the one-step detection of Salmonella enterica by using gold nanoparticle triggered enzyme signal amplification and magnetic separation. This immunosensor shows an enhanced sensitivity compared with an enzyme-linked immunosorbent assay, without the involvement of any equipment, thus providing a promising platform for rapid and sensitive detection of pathogens.