Issue 2, 2015

Multiplexed DNA detection based on positional encoding/decoding with self-assembled DNA nanostructures

Abstract

Current multiplexed analysis methods suffer from either slow reaction kinetics (planar arrays) or complicated encoding/decoding procedures (suspension arrays). We report herein a multiplexed DNA detection strategy that addresses these issues, based on positional encoding/decoding with self-assembled DNA nanostructures. The strategy enables the acquisition of high-resolution, consistent, and quantitative assay results in a single round of a transmission electron microscopy imaging operation. Applications in polymerase chain reaction-free settings and assays of other structurally distinct targets can be anticipated through the implementation of the strategy with miniaturized femtoliter/attoliter dispensing technology and readily accessible DNA conjugate structures.

Graphical abstract: Multiplexed DNA detection based on positional encoding/decoding with self-assembled DNA nanostructures

Supplementary files

Article information

Article type
Edge Article
Submitted
03 Sep 2014
Accepted
15 Oct 2014
First published
15 Oct 2014
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2015,6, 930-934

Author version available

Multiplexed DNA detection based on positional encoding/decoding with self-assembled DNA nanostructures

S. Sun, H. Yao, F. Zhang and J. Zhu, Chem. Sci., 2015, 6, 930 DOI: 10.1039/C4SC02696A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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