Issue 8, 2015

Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

Abstract

Fluorescent dyes have become increasingly important in cell biology since they enable high signal-to-noise and selectivity in visualizing subcellular organelles. Photoactivatable dyes allow for tracking and monitoring of a subset of cells or organelles. Here, we report the synthesis and application of a new class of large Stokes shift fluorescent dyes that are water-soluble, cell permeable, non-cytotoxic, and lysosome-specific. Additionally, we demonstrate temporally controlled sequential photoactivation of individual cells in close spatial proximity.

Graphical abstract: Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

Supplementary files

Article information

Article type
Edge Article
Submitted
02 May 2015
Accepted
09 Jun 2015
First published
17 Jun 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2015,6, 4508-4512

Author version available

Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

M. N. Tran, R. F. Rarig and D. M. Chenoweth, Chem. Sci., 2015, 6, 4508 DOI: 10.1039/C5SC01601K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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