3,6-Bis(1-methyl-4-vinylpyridinium)-carbazole diiodide as a marker for tracking living neural stem/precursor cells

Abstract

Until now, there has been no appropriate marker to specifically and effectively label living neural stem/precursor cells (NSPCs) to investigate NSPC migration and differentiation. Therefore, the purpose of this study was to develop a specific method for tracking NSPCs by a cell marker, 3,6-bis(1-methyl-4-vinylpyridinium)-carbazole diiodide (BMVC). It was found that the bright fluorescence spots could be rapidly observed in the nuclei of NSPCs, isolated from the embryonic rat cerebral cortex, after incubation with BMVC for just 5 min and could be maintained for exceeding 7 days without obvious decay. Even NSPCs were induced to undergo differentiation; their daughter cells also expressed bright fluorescence spots. In contrast, foreskin fibroblasts, adipose-derived stem cells (ADSCs), bone marrow mesenchymal stem cells (BMMSCs), neurons, and glial cells did not express the bright fluorescence spot in the presence of BMVC. In addition, BMVC-labeled NSPCs could be tracked in wound healing and decellularized rat brain models with minimal cell toxicity. Therefore, BMVC possessed a specific function to distinguish between NSPCs and other stem and neuron-related cells. These results are very encouraging, since a cell marker favorable for long-term labeling NSPCs should be useful in the development of strategies for tracking the development of NSPCs in neuroscience research.