Facile synthesis of N-halamine-labeled silica–polyacrylamide multilayer core–shell nanoparticles for antibacterial ability

Abstract

Silica–polymer antimicrobial composites with a core–shell nanostructure are often prepared through a polymeric process. However, it is difficult to control the polymerization degree of the polymers to give a uniform size distribution. In this article, we present a facile approach to produce antimicrobial silica@polyacrylamide (SiO₂@PAM) core–shell nanoparticles, which were synthesized via an electrostatic self-assembly method using acyclic N-halamine polymeric polyacrylamide. The morphologies and structures of these as-prepared nanoparticles were characterized by different techniques. And their antibacterial performance against both Gram-positive bacteria and Gram-negative bacteria was also evaluated. Based on the preliminary results, these core–shell nanosized spheres were made of an outer polymer shell which decorated the inner SiO₂ core, showing the encapsulation of silica nanoparticles with PAM polymers. After chlorination, the resultant nanosized particles displayed a powerful and stable bactericidal capability toward both of the two model bacterial species. Bactericidal assessment further suggested a coordinated effect of the well-known antibacterial performance of N-halamines and the flocculation of PAM on the antibacterial behavior. The in vitro cytotoxicity of the prepared nanoparticles with varying concentrations was studied using mouse fibroblast cells (L929). The CCK-8 assay revealed that the SiO₂@PAM composites possessed a non-cytotoxic and favorable response to the seeded cells in vitro. These results indicate the suitability of the SiO₂@PAM composite particles for controlling biocidal activity, demonstrating their potential applications in deactivating bacteria or even disease control.