Delivery of AIB1 siRNA by Ca2+/PEI/heparin composite nanoparticles effectively inhibits the growth of human breast cancer

Abstract

Here, a novel carrier fabricated by the interaction of negatively charged heparin and positively charged PEI and Ca²⁺ was investigated to deliver AIB1 siRNA into breast cancer cells both in vitro and in vivo. Ca²⁺/PEI/heparin nanoparticles were prepared by simply mixing heparin, PEI and CaCl₂ aqueous solution. Heparin in the Ca²⁺/PEI/heparin nanoparticles (40.9% heparin, w/w) decreased the cytotoxicity of PEI. According to the MTT assay, Ca²⁺/PEI/heparin NPs are superior to commercial Lipofectamine 2000 considering the safety. The Ca²⁺/PEI/heparin NPs are able to deliver siAIB1 into breast cancer cells as effectively as Lipofectamine 2000 both in vitro and in vivo. The in vivo experiment also indicated that the NF-κB/BCL-2 signal pathway might be the downstream signal pathway of AIB1 in regulating breast cancer proliferation and progression.