Issue 4, 2016
From the journal:

Analyst

A shelf-stable fluorogenic isothermal amplification assay for the detection of Burkholderia pseudomallei†

Jilien Michelle Wong Tzelinga  and  Chan Yean Yean*ab  

* Corresponding authors

a Department of Medical Microbiology & Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
E-mail: jilienwong@hotmail.com, yeancyn@yahoo.com
Tel: +609 7676258

b Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia

Abstract

A shelf-stable loop-mediated isothermal amplification (LAMP) reagent for Burkholderia pseudomallei detection is described. The coupling of LAMP reagents with the indirect colorimetric indicator and consequently its lyophilization enable the simple evaluation of results without the need for any advance laboratory instruments. The reagents were found to have a stable shelf life of at least 30 days with well-maintained sensitivity and specificity.

Graphical abstract: A shelf-stable fluorogenic isothermal amplification assay for the detection of Burkholderia pseudomallei

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Article information

DOI
https://doi.org/10.1039/C5AN01741F
Article type
Communication
Submitted
26 Aug 2015
Accepted
27 Dec 2015
First published
19 Jan 2016

Analyst, 2016,141, 1246-1249

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A shelf-stable fluorogenic isothermal amplification assay for the detection of Burkholderia pseudomallei

J. Michelle Wong Tzeling and C. Yean Yean, Analyst, 2016, 141, 1246 DOI: 10.1039/C5AN01741F

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