Issue 13, 2016

Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

Abstract

Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium–silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.

Graphical abstract: Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

Supplementary files

Article information

Article type
Communication
Submitted
15 Dec 2015
Accepted
19 Jan 2016
First published
19 Jan 2016
This article is Open Access
Creative Commons BY license

Analyst, 2016,141, 3982-3984

Author version available

Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

G. Faccio, M. B. Bannwarth, C. Schulenburg, V. Steffen, D. Jankowska, M. Pohl, R. M. Rossi, K. Maniura-Weber, L. F. Boesel and M. Richter, Analyst, 2016, 141, 3982 DOI: 10.1039/C5AN02573G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements