Issue 13, 2016

Modular calibrant sets for the structural analysis of nucleic acids by ion mobility spectrometry mass spectrometry

Abstract

This study explored the use of modular nucleic acid (NA) standards to generate calibration curves capable of translating primary ion mobility readouts into corresponding collision cross section (CCS) data. Putative calibrants consisted of single- (ss) and double-stranded (ds) oligo-deoxynucleotides reaching up to ∼40 kDa in size (i.e., 64 bp) and ∼5700 Å2 in CCS. To ensure self-consistency among reference CCS values, computational data obtained in house were preferred to any experimental or computational data from disparate sources. Such values were obtained by molecular dynamics (MD) simulations and either the exact hard sphere scattering (EHSS) or the projection superposition approximation (PSA) methods, and then plotted against the corresponding experimental values to generate separate calibration curves. Their performance was evaluated on the basis of their correlation coefficients and ability to provide values that matched the CCS of selected test samples mimicking typical unknowns. The results indicated that the predictive power benefited from the exclusion of higher charged species that were more susceptible to the destabilizing effects of Coulombic repulsion. The results revealed discrepancies between EHSS and PSA data that were ascribable to the different approximations used to describe the ion mobility process. Within the boundaries defined by these approximations and the challenges of modeling NA structure in a solvent-free environment, the calibrant sets enabled the experimental determination of CCS with excellent reproducibility (precision) and error (accuracy), which will support the analysis of progressively larger NA samples of biological significance.

Graphical abstract: Modular calibrant sets for the structural analysis of nucleic acids by ion mobility spectrometry mass spectrometry

Supplementary files

Article information

Article type
Paper
Submitted
23 Feb 2016
Accepted
28 Apr 2016
First published
29 Apr 2016

Analyst, 2016,141, 4084-4099

Modular calibrant sets for the structural analysis of nucleic acids by ion mobility spectrometry mass spectrometry

J. L. Lippens, S. V. Ranganathan, R. J. D'Esposito and D. Fabris, Analyst, 2016, 141, 4084 DOI: 10.1039/C6AN00453A

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