Issue 10, 2016
From the journal:

Analyst

Rapid and enzyme-free nucleic acid detection based on exponential hairpin assembly in complex biological fluids†

Cuiping Ma,ab   Menghua Zhang,ab   Shan Chen,c   Chao Liangab  and  Chao Shi*ab  

* Corresponding authors

a Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China
E-mail: sc169@163.com
Fax: (+86) 532-8402-2680
Tel: (+86) 532-8402-2680

b Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China
E-mail: mcp169@163.com
Fax: (+86) 532-8402-2680
Tel: (+86) 532-8402-2680

c College of Food Science and Engineering, Northwest Agriculture and Forestry University, Xianyang 712100, PR China

Abstract

Herein, we have developed a rapid and enzyme-free nucleic acid amplification detection method that combined the exponential self-assembly of four DNA hairpins and the FRET pair Cy3 and Cy5. This strategy was very ingenious and rapid, and could detect nucleic acids at concentrations as low as 10 pM in 15 min in biological fluids.

Graphical abstract: Rapid and enzyme-free nucleic acid detection based on exponential hairpin assembly in complex biological fluids

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C6AN00474A
Article type
Communication
Submitted
26 Feb 2016
Accepted
19 Apr 2016
First published
20 Apr 2016

Analyst, 2016,141, 2883-2886

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Rapid and enzyme-free nucleic acid detection based on exponential hairpin assembly in complex biological fluids

C. Ma, M. Zhang, S. Chen, C. Liang and C. Shi, Analyst, 2016, 141, 2883 DOI: 10.1039/C6AN00474A

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