Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system

Takeshi Komatsu; Saeed Mohammadi; Lori Shayne Alamo Busa; Masatoshi Maeki; Akihiko Ishida; Hirofumi Tani; Manabu Tokeshi

doi:10.1039/C6AN01409G

Image analysis for a microfluidic paper-based analytical device using the CIE Lab* color system†

Takeshi Komatsu,^a Saeed Mohammadi,^a Lori Shayne Alamo Busa,‡^a Masatoshi Maeki,^b Akihiko Ishida,*^b Hirofumi Tani^b and Manabu Tokeshi*^bcde

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* Corresponding authors

^a Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi8, Kita-ku, Sapporo 060-8628, Japan

^b Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan
E-mail: ishida-a@eng.hokudai.ac.jp
Fax: +81-11-706-6745
Tel: +81-11-706-6746

^c ImPACT Research Centre for Advanced Nanobiodevices, Nagoya University, Furo-cho Chikusa-ku, Nagoya 464-8603, Japan

^d Innovative Research Centre for Preventive Medical Engineering, Nagoya University, Furo-cho Chikusa-ku, Nagoya 464-8603, Japan

^e Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
E-mail: tokeshi@eng.hokudai.ac.jp
Fax: +81-11-706-6745
Tel: +81-11-706-6744

Abstract

The combination of a microfluidic paper-based analytical device (μPAD) and digital image analysis is widely used for quantitative analysis with μPADs because of its easy and simple operation. Herein, we have demonstrated a quantitative analysis based on multiple color changes on a μPAD. The CIE L*a*b* color system was employed to analyse the digital images obtained with the μPAD. We made pH measurements using a universal pH-indicator showing multiple color changes for various pH values of aqueous test solutions. The detectable pH range of this method was wider than the typical grayscale-based image analysis, and we succeeded in the measurements for a wide pH range of 2–9.

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Article information

DOI: https://doi.org/10.1039/C6AN01409G
Article type: Communication
Submitted: 21 Jun 2016
Accepted: 27 Oct 2016
First published: 28 Oct 2016
This article is Open Access

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Analyst, 2016,141, 6507-6509

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Image analysis for a microfluidic paper-based analytical device using the CIE Lab* color system

T. Komatsu, S. Mohammadi, L. S. A. Busa, M. Maeki, A. Ishida, H. Tani and M. Tokeshi, Analyst, 2016, 141, 6507 DOI: 10.1039/C6AN01409G

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