Early diagnosis of myocardial infarction in clinic through CK-MB detection using magnetic separation integrated with chemiluminescence

Abstract

We present herein a simple, sensitive and specific method for CK-MB detection for the early diagnosis of myocardial infarction through magnetic separation integrated with chemiluminescence. FITC and ABEI labelled anti-CK-MB antibodies and magnetic beads functionalized with anti-FITC antibodies are used to make a CK-MB complex after magnetic separation and the intensity of the chemiluminescence is used as a readout signal. The limit of detection is as low as 0.296 ng mL⁻¹ in serum and other common substances in serum do not cause interference. We test 120 real clinical samples using our method and the results are consistent with the existing commercial platform, which confirms the practical promise of our proposed method.