Simultaneous detection of fifteen biogenic amines in animal derived products by HPLC-FLD with solid-phase extraction after derivatization with dansyl chloride

Abstract

Simultaneous detection of many kinds of biogenic amines (BAs) is difficult because they have diverse structures. A HPLC method was established suitably for the simultaneous detection of fifteen biogenic amines in four types of animal-derived food products. The biogenic amines were derivatized with dansyl chloride, purified by using a Waters Sep-Pak C18 then separated on an ODS-2 Hypersil C18 column with a binary system using gradient elution. The derivatives were detected using wavelengths of 350 and 480 nm for excitation and emission, respectively. The limit of detection (LOD) for BAs ranged from 0.002 to 0.03 mg kg⁻¹ and the linearities of linear regression equations for fifteen biogenic amines were good (R² between 0.9990 and 0.9999). The method was applied to detect BAs in pork, beef, carp and crucian carp. Recoveries ranged from 70.49 to 121.16% at three spiked levels (0.5, 1 and 2 mg kg⁻¹), with RSDs in a range of 0.71–15.99%. Intra- and inter-day precisions (RSD%) were in a range of 0.30–4.60% and 4.62–14.97%, respectively. These data indicated that the established method was capable of simultaneous and precise quantitation of fifteen biogenic amines in animal-derived products of potential physiological importance for human health.