Issue 42, 2016

In vitro physiological performance factors of a catalase-based biosensor for real-time electrochemical detection of brain hydrogen peroxide in freely-moving animals

Abstract

Physiological performance factors of a catalase-based paired microelectrochemical biosensor, developed for real-time neurochemical monitoring of hydrogen peroxide (H2O2), were determined in the in vitro environment. The excellent ascorbic acid (AA) rejection characteristics and high sensitivity of the paired H2O2 sensor were assessed and verified. The highly suitable response time of the H2O2 sensor was demonstrated and the limit of detection of this sensor was calculated as ca. 0.075 μM. The H2O2 sensor was selective over the electroactive substance Mercaptosuccinate which is used in vivo to disrupt the enzymatic degradation of brain H2O2. The H2O2 sensing element of this paired design was impervious to an acidic/basic shift in environmental pH (6.5/8.0) relative to physiological levels (7.4). The influence of a temperature transition (ca. 23 °C to 37 °C) and a physiological temperature fluctuation expected to be found in vivo (1–4 °C), on the response of the paired H2O2 sensor was deemed negligible and consistent with other amperometric methods. The enzymatic component of the paired H2O2 sensor was found to be stable over a 14 day period of continuous ex vivo brain tissue exposure.

Graphical abstract: In vitro physiological performance factors of a catalase-based biosensor for real-time electrochemical detection of brain hydrogen peroxide in freely-moving animals

Supplementary files

Article information

Article type
Paper
Submitted
02 Aug 2016
Accepted
10 Oct 2016
First published
12 Oct 2016

Anal. Methods, 2016,8, 7614-7622

In vitro physiological performance factors of a catalase-based biosensor for real-time electrochemical detection of brain hydrogen peroxide in freely-moving animals

S. L. O'Riordan, K. Mc Laughlin and J. P. Lowry, Anal. Methods, 2016, 8, 7614 DOI: 10.1039/C6AY02190E

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