7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein

Munefumi Tokugawa; Yoshiaki Masaki; Jan Christian Canggadibrata; Kazuhei Kaneko; Takashi Shiozawa; Takashi Kanamori; Morten Grøtli; L. Marcus Wilhelmsson; Mitsuo Sekine; Kohji Seio

doi:10.1039/C5CC09700B

7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein†

Munefumi Tokugawa,^a Yoshiaki Masaki,^a Jan Christian Canggadibrata,^a Kazuhei Kaneko,^a Takashi Shiozawa,^a Takashi Kanamori,^a Morten Grøtli,^b L. Marcus Wilhelmsson,^c Mitsuo Sekine^a and Kohji Seio*^a

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* Corresponding authors

^a Department of Life Science, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan
E-mail: kseio@bio.titech.ac.jp

^b Department of Chemistry and Molecular Biology, University of Gothenburg, S-41296 Gothenburg, Sweden

^c Department of Chemistry and Chemical Engineering/Chemistry and Biochemistry, Chalmers University of Technology, S-41296 Gothenburg, Sweden

Abstract

7-(Benzofuran-2-yl)-7-deazadeoxyguanosine (^BFdG) was synthesized and incorporated into an oligodeoxynucleotide (ODN). The single-stranded ODN containing ^BFdG shows 91-fold fluorescence enhancement upon binding of single-strand DNA binding protein.

Chemical Communications

7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein†

Abstract

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7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein

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