Issue 17, 2016

Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins

Abstract

This work describes a general methodology for enhanced enzyme-linked immunosorbent assay (ELISA) that integrates enzyme-coated microparticle probes for robust yet highly efficient signal amplification and a handheld pH meter for a simple, portable, quantitative readout. Its utility is well demonstrated with the detection of the target protein with a 14-fold enhancement of sensitivity in comparison with the conventional optical ELISA.

Graphical abstract: Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins

Supplementary files

Article information

Article type
Communication
Submitted
29 Nov 2015
Accepted
09 Dec 2015
First published
09 Dec 2015

Chem. Commun., 2016,52, 3474-3477

Author version available

Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins

Y. Zhang, J. Yang, J. Nie, J. Yang, D. Gao, L. Zhang and J. Li, Chem. Commun., 2016, 52, 3474 DOI: 10.1039/C5CC09852A

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