Issue 27, 2016
From the journal:

Chemical Communications

Imidazolium-tagged glycan probes for non-covalent labeling of live cells†

David Benito-Alifonso,a   Shirley Tremell,a   Joanna C. Sadler,a   Monica Berry*b  and  M. Carmen Galan*a  

* Corresponding authors

a School of Chemistry, University of Bristol, Cantock's Close, UK
E-mail: M.C.Galan@bristol.ac.uk

b School of Physics, University of Bristol, NSQI, Tyndall Ave, Bristol BS8 1F, UK
E-mail: mon.berry@bristol.ac.uk

Abstract

Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.

Graphical abstract: Imidazolium-tagged glycan probes for non-covalent labeling of live cells

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Article information

DOI
https://doi.org/10.1039/C5CC10040B
Article type
Communication
Submitted
06 Dec 2015
Accepted
07 Mar 2016
First published
07 Mar 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2016,52, 4906-4909

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Imidazolium-tagged glycan probes for non-covalent labeling of live cells

D. Benito-Alifonso, S. Tremell, J. C. Sadler, M. Berry and M. C. Galan, Chem. Commun., 2016, 52, 4906 DOI: 10.1039/C5CC10040B

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