Issue 39, 2016
From the journal:

Chemical Communications

Thiolated uridine substrates and templates improve the rate and fidelity of ribozyme-catalyzed RNA copying†

Noam Prywes,‡ab   Yale S. Michaels,‡ac   Ayan Pal,ad   Seung Soo Oh ORCID logo ad  and  Jack W. Szostak*abd  

* Corresponding authors

a Howard Hughes Medical Institute, Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA

b Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, Massachusetts 02138, USA

c Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, UK

d Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
E-mail: szostak@molbio.mgh.harvard.edu

Abstract

Ribozyme-catalyzed RNA polymerization is inefficient and error prone. Here we demonstrate that two alternative bases, 2-thio-uridine (s2U) and 2-thio-ribo-thymidine (s2T), improve the rate and fidelity of ribozyme catalyzed nucleotide addition as NTP substrates and as template bases. We also demonstrate the functionality of s2U and s2T-containing ribozymes.

Graphical abstract: Thiolated uridine substrates and templates improve the rate and fidelity of ribozyme-catalyzed RNA copying

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Article information

DOI
https://doi.org/10.1039/C6CC02692C
Article type
Communication
Submitted
30 Mar 2016
Accepted
19 Apr 2016
First published
19 Apr 2016

Chem. Commun., 2016,52, 6529-6532

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Thiolated uridine substrates and templates improve the rate and fidelity of ribozyme-catalyzed RNA copying

N. Prywes, Y. S. Michaels, A. Pal, S. S. Oh and J. W. Szostak, Chem. Commun., 2016, 52, 6529 DOI: 10.1039/C6CC02692C

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