Issue 67, 2016

3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

Abstract

Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein–ligand complexes.

Graphical abstract: 3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

Supplementary files

Article information

Article type
Communication
Submitted
04 Jul 2016
Accepted
20 Jul 2016
First published
20 Jul 2016

Chem. Commun., 2016,52, 10237-10240

3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

B. Pan, F. Yang, Y. Ye, Q. Wu, C. Li, T. Huber and X. Su, Chem. Commun., 2016, 52, 10237 DOI: 10.1039/C6CC05490K

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