An efficient two-photon fluorescent probe for monitoring mitochondrial singlet oxygen in tissues during photodynamic therapy

Abstract

A promising two-photon fluorescent probe MNAH for detecting ¹O₂ during the PDT process in mitochondria was proposed for the first time. MNAH was successfully applied for two-photon imaging of ¹O₂ in living cells and tissues during the PDT process with deep-tissue imaging depth. MNAH can be a powerful molecular tool for studying ¹O₂ generation in mitochondria during the PDT process.