Issue 5, 2016
From the journal:

Catalysis Science & Technology

A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards α,ω-dinitriles†

Zhongyi Cheng,‡a   Wenjing Cui,‡a   Zhongmei Liu,a   Li Zhou,a   Min Wang,b   Michihiko Kobayashi*c  and  Zhemin Zhou*a  

* Corresponding authors

a Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China
E-mail: zhmzhou@jiangnan.edu.cn

b College of Biotechnology, Tianjin University of Science & Technology, No. 29 at 13th street TEDA, Tianjin 300457, PR China

c Institute of Applied Biochemistry and the Graduate School of Life and Environmental Sciences, The University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8572, Japan
E-mail: kobayashi.m.fe@u.tsukuba.ac.jp

Abstract

The β37 residue of nitrile hydratase (NHase) from Pseudomonas putida and NHase from Comamonas testosteroni played a critical role in directing enzyme regioselectivity. Amino acid substitution in this site modulated or even inverted enzyme regioselectivity towards aliphatic α,ω-dinitriles.

Graphical abstract: A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards α,ω-dinitriles

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Article information

DOI
https://doi.org/10.1039/C5CY01997D
Article type
Communication
Submitted
20 Nov 2015
Accepted
15 Jan 2016
First published
18 Jan 2016

Catal. Sci. Technol., 2016,6, 1292-1296

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A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards α,ω-dinitriles

Z. Cheng, W. Cui, Z. Liu, L. Zhou, M. Wang, M. Kobayashi and Z. Zhou, Catal. Sci. Technol., 2016, 6, 1292 DOI: 10.1039/C5CY01997D

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