Issue 6, 2016

Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

Abstract

Acetoin (AC) is regarded as one of the top potential sugar-derived chemical building blocks that can be used as food additives, precursors in chemical synthesis, and plant growth promoting molecules. In this study, a low-cost lignocellulosic resource of pretreated corn stover was used as a carbon source to produce AC. After redirecting the metabolic flux, fine tuning reducing power, and eliminating carbon catabolite repression in Enterobacter cloacae SDM, a systematically engineered strain SDM 53 was constructed, which is able to utilize glucose and xylose efficiently and simultaneously. Using fed-batch fermentation of SDM 53, 45.6 g L−1 AC was produced at a rate of 1.52 g L−1 h−1 using the lignocellulosic hydrolysate. Biotechnological synthesis of AC has various advantages such as being sustainable and environment-friendly. With its desirable properties, the engineered strain SDM 53 may be a potential choice for the industrial production of AC.

Graphical abstract: Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

Supplementary files

Article information

Article type
Paper
Submitted
18 Jul 2015
Accepted
21 Oct 2015
First published
21 Oct 2015

Green Chem., 2016,18, 1560-1570

Author version available

Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

L. Zhang, Q. Liu, Y. Ge, L. Li, C. Gao, P. Xu and C. Ma, Green Chem., 2016, 18, 1560 DOI: 10.1039/C5GC01638J

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