Issue 13, 2016
From the journal:

Lab on a Chip

Turning the corner in fertility: high DNA integrity of boundary-following sperm†

Lise Eamer,‡a   Marion Vollmer,‡a   Reza Nosrati,‡a   Maria C. San Gabriel,b   Krista Zeidan,b   Armand Zinib  and  David Sinton*a  

* Corresponding authors

a Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Rd., Toronto, ON M5S 3G8, Canada
E-mail: sinton@mie.utoronto.ca

b Department of Surgery, Urology Research Laboratory, McGill University and Research Institute, McGill University Health Centre, Montreal, QC H4A 3J1, Canada

Abstract

We present a passive microfluidic sperm selection strategy that collects motile sperm based on their preference to follow boundaries and turn corners. Clinical assessment of selected human sperm from the device revealed a strong correlation between high DNA integrity and the tendency for sperm to follow boundaries. Human sperm with preference to follow boundaries on the left- or right-hand sides have higher (>51%) DNA integrity than straight swimmers and significantly higher (>67%) DNA integrity than sperm in raw semen. Boundary following behaviour offers a strategy to selecting sperm with the highest DNA integrity to improve the success rate of assisted reproduction.

Graphical abstract: Turning the corner in fertility: high DNA integrity of boundary-following sperm

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Article information

DOI
https://doi.org/10.1039/C6LC00490C
Article type
Communication
Submitted
12 Apr 2016
Accepted
23 May 2016
First published
23 May 2016

Lab Chip, 2016,16, 2418-2422

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Turning the corner in fertility: high DNA integrity of boundary-following sperm

L. Eamer, M. Vollmer, R. Nosrati, M. C. San Gabriel, K. Zeidan, A. Zini and D. Sinton, Lab Chip, 2016, 16, 2418 DOI: 10.1039/C6LC00490C

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