Synthesis of triazole-linked morpholino oligonucleotides via CuI catalysed cycloaddition

Abstract

Triazole-linked morpholino (^TLMO) oligonucleic acids were synthesised using the Cu^I catalysed (3 + 2) azide–alkyne cycloaddition (CuAAC) reaction. The modified DNA analogues were incorporated into 13-mer sequences via solid phase synthesis. UV melting experiments showed that the ^TLMO modification gives higher T_m values than the corresponding ^TLDNA modification.