Issue 23, 2016

Synthesis of tumor necrosis factor α for use as a mirror-image phage display target

Abstract

Tumor Necrosis Factor alpha (TNFα) is an inflammatory cytokine that plays a central role in the pathogenesis of chronic inflammatory disease. Here we describe the chemical synthesis of L-TNFα along with the mirror-image D-protein for use as a phage display target. The synthetic strategy utilized native chemical ligation and desulfurization to unite three peptide segments, followed by oxidative folding to assemble the 52 kDa homotrimeric protein. This synthesis represents the foundational step for discovering an inhibitory D-peptide with the potential to improve current anti-TNFα therapeutic strategies.

Graphical abstract: Synthesis of tumor necrosis factor α for use as a mirror-image phage display target

Supplementary files

Article information

Article type
Paper
Submitted
18 Apr 2016
Accepted
16 May 2016
First published
16 May 2016

Org. Biomol. Chem., 2016,14, 5298-5303

Author version available

Synthesis of tumor necrosis factor α for use as a mirror-image phage display target

M. E. Petersen, M. T. Jacobsen and M. S. Kay, Org. Biomol. Chem., 2016, 14, 5298 DOI: 10.1039/C6OB00824K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements