The fluorescence detection of autophagosomes in live cells under starvation using core-substituted naphthalenediimide probes

Abstract

A naphthalenediimide colorimetric pH sensor was designed, synthesised and employed for live cell applications. In DMSO : buffer solution, the NDI probe exhibits good pH selectivity and high photostability. We used a core-substituted naphthalenediimide (NDI-C) probe for the analysis of the autophagosomes. A new flow cytometric method was also developed for the analysis of cell population on the basis of intracellular pH.