Issue 10, 2016

Early release of 1-pyrroline by Pseudomonas aeruginosa cultures discovered using ambient corona discharge ionization mass spectrometry

Abstract

Pseudomonas aeruginosa (PA) is a leading cause of nosocomial infections in humans with an increasing number of health-threatening implications, which urges faster clinical detection of this pathogen. In the present study, we discovered for the first time the early release of 1-pyrroline vapor by PA cultures using direct ambient mass spectrometry (AMS) analysis of the bacterial culture headspace based on corona discharge ionization. Importantly, the concentration of 1-pyrroline in PA cultures was found to greatly increase during the lag phase and early log phase of bacterial growth (3–6 h, 200–800 ppb), enabling early detection. Typically, 1-pyrroline produced by PA cultures could already be detected in our experiments after 0.5–4 h of incubation at the clinically relevant initial bacterial counts. A reference AMS screen of common infectious microbes from other genera, including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Escherichia coli, Klebsiella pneumonia, Acinetobacter baumannii, Enterococcus faecalis, Klebsiella oxytoca, Candida albicans, Candida tropicalis, Candida parapsilosis, Enterococcus faecalis, Enterobacter cloacae, did not reveal a notable release of 1-pyrroline. Our results indicate the high suitability of volatile 1-pyrroline for the early and reliable diagnosis of Pseudomonas infections using commonly available MS instrumentation.

Graphical abstract: Early release of 1-pyrroline by Pseudomonas aeruginosa cultures discovered using ambient corona discharge ionization mass spectrometry

Supplementary files

Article information

Article type
Paper
Submitted
20 Nov 2015
Accepted
01 Jan 2016
First published
06 Jan 2016

RSC Adv., 2016,6, 8449-8455

Author version available

Early release of 1-pyrroline by Pseudomonas aeruginosa cultures discovered using ambient corona discharge ionization mass spectrometry

L. Hu, J. Liang, K. Chingin, Y. Hang, X. Wu and H. Chen, RSC Adv., 2016, 6, 8449 DOI: 10.1039/C5RA24594J

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