Issue 34, 2016, Issue in Progress

Enhanced binding capacity of boronate affinity fibrous material for effective enrichment of nucleosides in urine samples

Abstract

In this work, polyethyleneimine-modified boronate affinity fibrous cotton with good selectivity and higher binding capacity for cis-diols was developed for in-pipette-tip solid-phase extraction (SPE). The introduction of polyethyleneimine for the modification of cotton fiber provided abundant binding sites for adsorption of cis-diols, resulting in higher binding capacities up to 300 μg g−1 for catechol, 450 μg g−1 for dopamine and 700 μg g−1 for adenosine, respectively. The adsorbent could still show recognition ability towards cis-diols even with a 1000-fold amount of interferences in the solution. Several extraction parameters were optimized, including adsorbent dose, pipette times, washing and elution solvents. Under optimal extraction conditions, the proposed in-pipette-tip SPE method coupled with reversed-phase liquid chromatography was successfully used to extract nucleosides in urine. The limit of detection was 3.5–4.7 ng mL−1 and the recoveries were in the range of 89–102% with relative standard deviations of less than 9.9%. In conclusion, combination of the specific adsorption performance of the prepared boronate affinity fibrous material and in-pipette-tip SPE provided a fast pretreatment method of trace cis-diols in complex real samples.

Graphical abstract: Enhanced binding capacity of boronate affinity fibrous material for effective enrichment of nucleosides in urine samples

Supplementary files

Article information

Article type
Paper
Submitted
28 Dec 2015
Accepted
04 Mar 2016
First published
07 Mar 2016

RSC Adv., 2016,6, 28470-28476

Enhanced binding capacity of boronate affinity fibrous material for effective enrichment of nucleosides in urine samples

L. Gao, C. Wang and Y. Wei, RSC Adv., 2016, 6, 28470 DOI: 10.1039/C5RA27898H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements