Issue 58, 2016, Issue in Progress
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RSC Advances

Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials†

F. M. P. Tonelli,ab   S. M. S. N. Lacerda,cd   N. C. O. Paiva,ab   M. S. Lemos,cd   A. C. de Jesus,e   F. G. Pacheco,f   J. D. Corrêa-Junior,g   L. O. Ladeira,e   C. A. Furtado,f   L. R. França*cd  and  R. R. Resende*ab  

* Corresponding authors

a Cell Signaling and Nanobiotechnology Laboratory, Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, Brazil

b Nanocell Institute, Divinópolis, Brazil
E-mail: rrresende@hotmail.com

c Cell Biology Laboratory, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Brazil
E-mail: lrfranca@icb.ufmg.br

d National Institute for Amazonian Research (INPA), Manaus, Brazil
E-mail: lrfranca@inpa.gov.br

e Nanomaterials Laboratory, Department of Physics and Center of Microscopy, Federal University of Minas Gerais, Belo Horizonte, Brazil

f Chemistry of Nanostructures Laboratory, Nuclear Technology Development Center, Belo Horizonte, Brazil

g Laboratory of Biological Interaction and Animal Reproduction, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Brazil

Abstract

Multiwalled carbon nanotubes (MWCNTs), nanographene oxide (NGO), and gold nanorods (NRs) can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested. Therefore, nanomaterials can contribute to achieve fish transgenesis.

Graphical abstract: Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials

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Article information

DOI
https://doi.org/10.1039/C6RA07010H
Article type
Communication
Submitted
16 Mar 2016
Accepted
23 May 2016
First published
25 May 2016

RSC Adv., 2016,6, 52636-52641

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Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials

F. M. P. Tonelli, S. M. S. N. Lacerda, N. C. O. Paiva, M. S. Lemos, A. C. de Jesus, F. G. Pacheco, J. D. Corrêa-Junior, L. O. Ladeira, C. A. Furtado, L. R. França and R. R. Resende, RSC Adv., 2016, 6, 52636 DOI: 10.1039/C6RA07010H

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