Issue 80, 2016
From the journal:

RSC Advances

Influence of high-mannose glycan whose glucose moiety is substituted with 5-thioglucose on calnexin/calreticulin cycle†

Masafumi Sakono,*ab   Akira Seko,a   Yoichi Takeda,ac   Masakazu Hachisu,ad   Akihiko Koizumi,ae   Kohki Fujikawa,af   Hideharu Setog  and  Yukishige Ito*ag  

* Corresponding authors

a Japan Science and Technology Agency (JST), ERATO Ito Glycotrilogy Project, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
E-mail: msakono@eng.u-toyama.ac.jp, yukito@riken.jp

b Department of Applied Chemistry, University of Toyama, 3190 Gofuku, Toyama, Toyama 930-855, Japan

c Department of Biotechnology, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga 525-8577, Japan

d Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan

e Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan

f SUNTORY Foundation for Life Sciences, Bioorganic Research Institute, 8-1-1 Seikadai Seika-cho, Soraku-gun, Kyoto 619-0284, Japan

g Synthetic Cellular Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Abstract

Our study first revealed that UDP-5-thioglucose functions as a glycosyl donor of UDP-glucose: glycoprotein glucosyltransferase to produce 5-thio-glucosylated Man9 (5S-G1M9). Subsequently, we observed that only calreticulin can interact with 5S-G1M9. Finally, the 5-thioglucose residue was resistant to hydrolysis by glucosidase II.

Graphical abstract: Influence of high-mannose glycan whose glucose moiety is substituted with 5-thioglucose on calnexin/calreticulin cycle

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Article information

DOI
https://doi.org/10.1039/C6RA16476E
Article type
Communication
Submitted
26 Jun 2016
Accepted
09 Aug 2016
First published
09 Aug 2016

RSC Adv., 2016,6, 76879-76882

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Influence of high-mannose glycan whose glucose moiety is substituted with 5-thioglucose on calnexin/calreticulin cycle

M. Sakono, A. Seko, Y. Takeda, M. Hachisu, A. Koizumi, K. Fujikawa, H. Seto and Y. Ito, RSC Adv., 2016, 6, 76879 DOI: 10.1039/C6RA16476E

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