SPECT imaging of cytochrome c in pressure overload mice hearts

Abstract

Clinically, pressure overload (PO) occurs in many clinical settings such as hypertension and valvular stenosis especially in the current aging society. During cardiac remodeling induced by PO, mitochondria were injured and cytochrome c was released. However, few studies have targeted cytochrome c for imaging. In this study, Technium-99m labeled cytochrome c antibody was developed to image PO hearts induced by transverse aortic constriction surgery. ^99mTc-Anti-cytochrome c was available to image and differentiate cytochrome c in normal mice hearts and PO hearts. The findings on images were consistent with detection by cardiac function and molecular biological assay. Hence, the noninvasive targeted cytochrome c imaging of mice hearts can be realized via ^99mTc-Anti-cytochrome c based SPECT.