Issue 115, 2016

Norbornene derived nanocarrier reduces isoniazid mediated liver toxicity: assessment in HepG2 cell line and zebrafish model

Abstract

The aim of this study was to investigate the protective effect of the stimuli-responsive norbornene-based nanocarrier complex of isoniazid, compared to pure isoniazid, on liver cells, by in vivo and in vitro methods. Hepatic damage induced in a zebrafish model by isoniazid resulted in significant alterations in liver histology, gene expression of drug metabolizing genes, reduced glutathione (GSH) levels, and DNA damage. An increased level of intracellular reactive oxygen species (ROS) was detected in HepG2 cells on exposure to isoniazid. Interestingly, the isoniazid conjugated nanocarrier exhibited a more protective effect on liver cells compared to isoniazid. A significant reduction in the cyp2p6 gene, elevation in GSH levels, reduced DNA damage, and a normal histological tissue pattern was observed in liver tissues exposed to the isoniazid conjugated nanocarrier. Also, the isoniazid conjugated nanocarrier showed negligible ROS generation and in vitro cytotoxicity in HepG2 cells. These encouraging results may prompt further research in the advance of norbornene-based nano systems in nanotherapeutics and biomedical applications.

Graphical abstract: Norbornene derived nanocarrier reduces isoniazid mediated liver toxicity: assessment in HepG2 cell line and zebrafish model

Supplementary files

Article information

Article type
Paper
Submitted
23 Sep 2016
Accepted
01 Dec 2016
First published
06 Dec 2016

RSC Adv., 2016,6, 114927-114936

Norbornene derived nanocarrier reduces isoniazid mediated liver toxicity: assessment in HepG2 cell line and zebrafish model

T. Anju, R. Preetha, R. Shunmugam, S. R. Mane, J. Arockiaraj and V. Kumaresan, RSC Adv., 2016, 6, 114927 DOI: 10.1039/C6RA23557C

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