Issue 9, 2017
From the journal:

Chemical Communications

High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis†

Haibo Jiang,*a   Melissa K. Passarelli,b   Peter M. G. Munro,c   Matt R. Kilburn,a   Andrew West,d   Colin T. Dollery,d   Ian S. Gilmoreb  and  Paulina D. Rakowska*b  

* Corresponding authors

a Centre for Microscopy, Characterisation and Analysis, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
E-mail: Haibo.Jiang@uwa.edu.au

b National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Hampton Road, Teddington, Middlesex, UK
E-mail: Paulina.Rakowska@npl.co.uk

c Institute of Ophthalmology, University College London, Bath Street, London, UK

d GlaxoSmithKline, Gunnels Wood Road, Stevenage, Hertfordshire, UK

Abstract

Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.

Graphical abstract: High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

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Article information

DOI
https://doi.org/10.1039/C6CC08549K
Article type
Communication
Submitted
24 Oct 2016
Accepted
07 Dec 2016
First published
07 Dec 2016

Chem. Commun., 2017,53, 1506-1509

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High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

H. Jiang, M. K. Passarelli, P. M. G. Munro, M. R. Kilburn, A. West, C. T. Dollery, I. S. Gilmore and P. D. Rakowska, Chem. Commun., 2017, 53, 1506 DOI: 10.1039/C6CC08549K

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