Issue 2, 2017
From the journal:

Chemical Communications

Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells†

Pedro M. S. D. Cal,ab   Florian Sieglitz,a   Fábio M. F. Santos,c   Cátia Parente Carvalho,d   Ana Guerreiro,a   Jean B. Bertoldo,b   Uwe Pischel,d   Pedro M. P. Gois*c  and  Gonçalo J. L. Bernardes ORCID logo *ab  

* Corresponding authors

a Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, Lisboa, Portugal
E-mail: gbernardes@medicina.ulisboa.pt

b Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK
E-mail: gb453@cam.ac.uk

c Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, Lisboa, Portugal
E-mail: pedrogois@ff.ul.pt

d CIQSO – Center for Research in Sustainable Chemistry and Department of Chemistry, University of Huelva, Campus de El Carmen s/n, 21071 Huelva, Spain

Abstract

Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells.

Graphical abstract: Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells

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Article information

DOI
https://doi.org/10.1039/C6CC08671C
Article type
Communication
Submitted
28 Oct 2016
Accepted
25 Nov 2016
First published
30 Nov 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 368-371

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Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells

P. M. S. D. Cal, F. Sieglitz, F. M. F. Santos, C. Parente Carvalho, A. Guerreiro, J. B. Bertoldo, U. Pischel, P. M. P. Gois and G. J. L. Bernardes, Chem. Commun., 2017, 53, 368 DOI: 10.1039/C6CC08671C

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