Issue 35, 2017

Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

Abstract

There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid–liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.

Graphical abstract: Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

Supplementary files

Article information

Article type
Communication
Submitted
17 Feb 2017
Accepted
23 Mar 2017
First published
23 Mar 2017

Chem. Commun., 2017,53, 4775-4778

Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

M. Sobrinos-Sanguino, S. Zorrilla, C. D. Keating, B. Monterroso and G. Rivas, Chem. Commun., 2017, 53, 4775 DOI: 10.1039/C7CC01289F

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