Supercharging SpyCatcher toward an intrinsically disordered protein with stimuli-responsive chemical reactivity

Abstract

We report a supercharged, intrinsically disordered protein, SpyCatcher(−), possessing stimuli-responsive reactivity toward SpyTag with tunable yields ranging from 4% to 98% depending on pH, temperature, ionic strength, etc. The CD and NMR studies reveal that the reaction occurs through a folded intermediate formed probably via a different mechanism from that of SpyCatcher.