Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes†
S.
Barreda-García, 
a
R.
Miranda-Castro,
a
N.
de-los-Santos-Álvarez,
a
A. J.
Miranda-Ordieres
a
and
M. J.
Lobo-Castañón
*a
a
R.
Miranda-Castro,
a
N.
de-los-Santos-Álvarez,
a
A. J.
Miranda-Ordieres
a
and
M. J.
Lobo-Castañón
*a
Abstract
An on-surface isothermal helicase-dependent amplification is devised for simple, point-of-need quantification of bacterial genomes. The method relies on the enzyme-extension of a thiol-modified reverse primer anchored to indium tin oxide electrodes, which shows strikingly high thermal and storage stability. Amplification and electrochemical detection of only 10 genomes are thus performed on the same platform without thermal cycling.
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