Issue 94, 2017
From the journal:

Chemical Communications

A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex†

Sarah H. Hewitt,a   Jonathan Parris,a   Romain Mailhota  and  Stephen J. Butler ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Loughborough University, Epinal Way, Loughborough, UK
E-mail: s.j.butler@lboro.ac.uk
Tel: +44 (0)1509 222577

Abstract

We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

Graphical abstract: A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

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Article information

DOI
https://doi.org/10.1039/C7CC05887J
Article type
Communication
Submitted
28 Jul 2017
Accepted
22 Sep 2017
First published
13 Nov 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 12626-12629

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A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

S. H. Hewitt, J. Parris, R. Mailhot and S. J. Butler, Chem. Commun., 2017, 53, 12626 DOI: 10.1039/C7CC05887J

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