Issue 87, 2017

Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Abstract

A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

Graphical abstract: Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Supplementary files

Article information

Article type
Communication
Submitted
06 Sep 2017
Accepted
11 Oct 2017
First published
16 Oct 2017

Chem. Commun., 2017,53, 11929-11932

Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

J. Lohse, A. Schindl, N. Danda, C. P. Williams, K. Kramer, B. Kuster, M. D. Witte and G. Médard, Chem. Commun., 2017, 53, 11929 DOI: 10.1039/C7CC07001B

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