Issue 3, 2017

Real-time detection and imaging of copper(ii) in cellular mitochondria

Abstract

A triphenylamine–pyridine derivative, viz., a fluorescence probe was designed and synthesized. The probe displayed an “on–off–on” fluorescence switch, upon the sequential addition of Cu2+ and ethylenediaminetetraacetic acid (EDTA). The detection mechanism was confirmed by using UV-vis, fluorescence and 1H NMR titration spectra. Interestingly, the probe can accumulate in mitochondria, and thus detect Cu2+ in real-time there, which indicated that probe L can be used to monitor intracellular mitochondrial Cu2+ levels. Further understanding of the biological functions of Cu2+ in mitochondria has important implications for treating cancer and other diseases.

Graphical abstract: Real-time detection and imaging of copper(ii) in cellular mitochondria

Supplementary files

Article information

Article type
Paper
Submitted
02 Nov 2016
Accepted
28 Nov 2016
First published
28 Nov 2016

Org. Biomol. Chem., 2017,15, 598-604

Real-time detection and imaging of copper(II) in cellular mitochondria

H. Li, R. Zhang, C. Li, B. Huang, T. Yu, X. Huang, X. Zhang, F. Li, H. Zhou and Y. Tian, Org. Biomol. Chem., 2017, 15, 598 DOI: 10.1039/C6OB02384C

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