Issue 13, 2017

Stereochemical investigations of the Tetrahymena cyclase, a model system for euphane/tirucallane biosynthesis

Abstract

The squalene cyclase of Tetrahymena pyriformis cyclizes 2,3-dihydrosqualene to euph-7-ene. This was used as a model for euphane biosynthesis. D-ring formation was demonstrated to involve pre-chair folding through an experiment with 2,3-dihydro-5-oxasqualene. This requires that a non-least motion rotation (120°) of the C17–20 bond in the intermediate deoxydammaranyl cation occurs before the first 1,2-hydride shift. Truncated substrates relieved the hindrance associated with this rotation and permitted a least motion pathway. Several triterpenes were found to be minor products of the Tetrahymena cyclase.

Graphical abstract: Stereochemical investigations of the Tetrahymena cyclase, a model system for euphane/tirucallane biosynthesis

Supplementary files

Article information

Article type
Paper
Submitted
07 Feb 2017
Accepted
07 Mar 2017
First published
13 Mar 2017

Org. Biomol. Chem., 2017,15, 2823-2830

Stereochemical investigations of the Tetrahymena cyclase, a model system for euphane/tirucallane biosynthesis

J. Giner and J. Feng, Org. Biomol. Chem., 2017, 15, 2823 DOI: 10.1039/C7OB00296C

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